ABSTRACT
Calciphylactic blockade of Reticulo endothelial system (REB) was used to study the role of reticulo-endothelial system (RES) in hepatic regeneration. REB was induced by i.v. egg yolk administration in DHT sensitized animals, which were subsequently partially hepatectomized. Mitotic index and percentage regeneration were studied in these animals 48 hr after partial hepatectomy. REB produced a significant depression of the mitotic index and percentage regeneration in regenerating livers. This suggests that functional integrity of the RES in essential hepatic parenchymal proliferation after partial hepatectomy.
Subject(s)
Animals , Carbon/metabolism , Colloids , Dihydrotachysterol/pharmacology , Hepatectomy , Liver/anatomy & histology , Liver Regeneration , Male , Mitosis , Mononuclear Phagocyte System/drug effects , Rats , Rats, Inbred StrainsABSTRACT
Intravenous I131 Rose bengal clearance was conducted in partially hepatectomized rats with a dose of 1 muCi/100 gm body weight. Blood samples were taken at regular intervals up to 25 minutes which were suitable diluted to count the remaining radioactivity. From these values t1/2 and the clearance constants were worked out upto a period of 120 hr after partial hepatectomy. An analysis of the results show that there is a linear correlation between the rose bengal clearance and the regenerating hepatic mass which may be used for quantitative assessment of functional hepatic mass.